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PIWIL1 antibody, Internal

Anti-PIWIL1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89021

Cat. No. GTX89021

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all PIWIL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 4-6μg/ml

Note :

IHC-P
Human Testis shows textured cytoplasm staining in all germ cells.
Not tested in other applications.

Calculated MW

99 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89021-PEP).

Product Note

This antibody is expected to recognise both reported isoforms (NP_004755.2; NP_001177900.1).

Predict Reactivity

Mouse, Rat, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-SNRKDKYDAIKKY, from the internal region of the protein sequence according to NP_004755.2; NP_001177900.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

piwi like RNA-mediated gene silencing 1 , CT80.1 , HIWI , MIWI , PIWI

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-PIWIL1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89021

GTX89021 IHC-P Image

IHC-P analysis of human testis using GTX89021 PIWIL1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 4μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399