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PIWIL3 antibody

Anti-PIWIL3 antibody used in Western Blot (WB). GTX85145
Anti-PIWIL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85145

Cat. No. GTX85145

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all PIWIL3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ICC/IF 20 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

101 kDa. ( Note )

Positive Control

3T3 (NIH) Cell Lysate(GTX27179) , 3T3/BALB Cell Slide

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PIWI-L3 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PIWI-L3.The immunogen is located within amino acids 130 - 180 of PIWI-L3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

piwi like RNA-mediated gene silencing 3 , HIWI3

Cellular Localization

Cytoplasm

Background

PIWI-L3 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. Recent studies have shown that overexpression of PIWI-L3 (as well as other PIWI-like proteins) are potential biomarkers for astrocytic glioma, meningioma, and other cancers.

Database

Research Area

DATA IMAGES

Anti-PIWIL3 antibody used in Western Blot (WB). GTX85145

GTX85145 WB Image

WB analysis of 3T3 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX85145 PIWIL3 antibody.
Working concentration : 1 μg/ml

Anti-PIWIL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85145

GTX85145 ICC/IF Image

ICC/IF analysis of 3T3 cells using GTX85145 PIWIL3 antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399