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PIWIL4 antibody [C2C3], C-term

Anti-PIWIL4 antibody [C2C3], C-term used in Western Blot (WB). GTX119387
Anti-PIWIL4 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119387
Anti-PIWIL4 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX119387

Cat. No. GTX119387

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PIWIL4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Predict Reactivity

Rat, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PIWIL4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617832

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

piwi like RNA-mediated gene silencing 4 , HIWI2 , MIWI2

Cellular Localization

Nucleus , Cytoplasm

Background

PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PIWIL4 antibody [C2C3], C-term used in Western Blot (WB). GTX119387

GTX119387 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIWIL4 antibody [C2C3], C-term (GTX119387) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PIWIL4 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119387

GTX119387 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using PIWIL4(GTX119387) antibody at 1:200 dilution.

Anti-PIWIL4 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX119387

GTX119387 IHC-P Image

Immunohistochemical analysis of paraffin-embedded 59T xenograft, using PIWIL4(GTX119387) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169