GeneTex
United States (US)

PKA 2 beta antibody

GTX105558_39890_IHC_19011506_170.jpg
GTX105558_39890_20170713_WB_M_R_19011506_891.jpg
GTX105558_39890_20171127_IHC-Fr_M_19011506_107.jpg
GTX105558_39890_IFA_19011506_813.jpg
GTX105558_WB_19011506_141.jpg

Cat No. GTX105558

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HeLa , HepG2 , mouse brain , rat brain

Predict Reactivity

Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

0.67mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKA 2 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PRKAR2 antibody, RII-BETA antibody, PRKAR2B antibody, cAMP-dependent protein kinase type II-beta regulatory subunit antibody, cAMP-dependent protein kinase type II-beta regulatory chain antibody, H_RG363E19.2 antibody, WUGSC:H_RG363E19.2 antibody, "protein kinase, cAMP-dependent, regulatory, type II, beta antibody"

Background

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX105558_39890_IHC_19011506_170.jpg

GTX105558 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PKA 2 beta(GTX105558) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

GTX105558_39890_20170713_WB_M_R_19011506_891.jpg

GTX105558 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKA 2 beta antibody (GTX105558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105558_39890_20171127_IHC-Fr_M_19011506_107.jpg

GTX105558 IHC-Fr Image

PKA 2 beta antibody detects PKA 2 beta protein by immunohistochemical analysis.
Samples: Frozen Sectioned adult mouse hippocampus.
Green: PKA 2 beta protein stained by PKA 2 beta antibody (GTX105558) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

GTX105558_39890_IFA_19011506_813.jpg

GTX105558 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PKA 2 beta(GTX105558) antibody at 1:200 dilution.

GTX105558_WB_19011506_141.jpg

GTX105558 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
10% SDS PAGE
GTX105558 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf