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PKA 2 beta antibody

Anti-PKA 2 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105558
Anti-PKA 2 beta antibody used in IHC (Frozen sections) (IHC-Fr). GTX105558
Anti-PKA 2 beta antibody used in Western Blot (WB). GTX105558
Anti-PKA 2 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX105558
Anti-PKA 2 beta antibody used in Western Blot (WB). GTX105558

Cat. No. GTX105558

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PKA 2 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HeLa , HepG2 , mouse brain , rat brain

Predict Reactivity

Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKA 2 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037699

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase cAMP-dependent type II regulatory subunit beta , PRKAR2 , RII-BETA

Background

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PKA 2 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105558

GTX105558 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PKA 2 beta(GTX105558) antibody at 1:200 dilution.

Anti-PKA 2 beta antibody used in IHC (Frozen sections) (IHC-Fr). GTX105558

GTX105558 IHC-Fr Image

PKA 2 beta antibody detects PKA 2 beta protein by immunohistochemical analysis.
Samples: Frozen Sectioned adult mouse hippocampus.
Green: PKA 2 beta protein stained by PKA 2 beta antibody (GTX105558) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-PKA 2 beta antibody used in Western Blot (WB). GTX105558

GTX105558 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
10% SDS PAGE
GTX105558 diluted at 1:1000

Anti-PKA 2 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX105558

GTX105558 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PKA 2 beta(GTX105558) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-PKA 2 beta antibody used in Western Blot (WB). GTX105558

GTX105558 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKA 2 beta antibody (GTX105558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169