PKA C alpha antibody [N2C3]

Name: PKA C alpha antibody [N2C3]
Brand: GeneTex
SKU: GTX104934
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-PKA C alpha antibody [N2C3] used in Western Blot (WB). GTX104934

Anti-PKA C alpha antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104934

Anti-PKA C alpha antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104934

Cat. No. GTX104934

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all PKA C alpha products

View product citations for antibody GTX104934 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Dog, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKA C alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037751

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase cAMP-activated catalytic subunit alpha , PKACA , PPNAD4

Cellular Localization

Cytoplasm , Nucleus

Background

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Database

Gene ID: 5566 PRKACA
UniProt: P17612 PRKACA

Research Area

DATA IMAGES

GTX104934 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX104934 diluted at 1:1000

GTX104934 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKA C alpha antibody [N2C3] (GTX104934) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104934 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon, using PKA alpha(GTX104934) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104934 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PKA alpha(GTX104934) antibody at 1:200 dilution.

REFERENCE

Application Reference

Park EJ et al. Nat Commun 2023; 14 (1) : 1097 Ssu72 phosphatase is essential for thermogenic adaptation by regulating cytosolic translation.

Application : WB

Tseng IC et al. J Pathol 2017; (Epub) Microinsertions in PRKACA cause activation of the protein kinase A pathway in cardiac myxoma.

Application : IHC-P

Reactivity : Human

Chang CY et al. Biomed Res Int 2015; 2015 : 159015 Subchronic Arsenic Exposure Induces Anxiety-Like Behaviors in Normal Mice and Enhances Depression-Like Behaviors in the Chemically Induced Mouse Model of Depression.

Application : WB

Reactivity : Mouse

Chu CS et al. J Biol Chem 2011; 286 (41) : 35843-51 Protein kinase A-mediated serine 35 phosphorylation dissociates histone H1.4 from mitotic chromosome.

Application : WB

Reactivity : Human

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