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PKA C alpha antibody [N2C3]

Anti-PKA C alpha antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104934
Anti-PKA C alpha antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104934
Anti-PKA C alpha antibody [N2C3] used in Western Blot (WB). GTX104934
Anti-PKA C alpha antibody [N2C3] used in Western Blot (WB). GTX104934

Cat No. GTX104934

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

HeLa , Jurkat , NT2D1 , mouse brain

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Dog, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKA C alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

protein kinase cAMP-activated catalytic subunit alpha , PKACA , PPNAD4

Cellular Localization

Cytoplasm , Nucleus

Background

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-PKA C alpha antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104934

GTX104934 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PKA alpha(GTX104934) antibody at 1:200 dilution.

Anti-PKA C alpha antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104934

GTX104934 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon, using PKA alpha(GTX104934) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PKA C alpha antibody [N2C3] used in Western Blot (WB). GTX104934

GTX104934 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX104934 diluted at 1:1000

Anti-PKA C alpha antibody [N2C3] used in Western Blot (WB). GTX104934

GTX104934 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKA C alpha antibody [N2C3] (GTX104934) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169