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PKA R2 antibody [4A3]

KO/KD Overexpression
Anti-PKA R2 antibody [4A3] used in IHC (Paraffin sections) (IHC-P). GTX83825
Anti-PKA R2 antibody [4A3] used in IHC (Paraffin sections) (IHC-P). GTX83825
Anti-PKA R2 antibody [4A3] used in IHC (Paraffin sections) (IHC-P). GTX83825
Anti-PKA R2 antibody [4A3] used in IHC (Paraffin sections) (IHC-P). GTX83825
Anti-PKA R2 antibody [4A3] used in IHC (Paraffin sections) (IHC-P). GTX83825
Anti-PKA R2 antibody [4A3] used in Western Blot (WB). GTX83825
Anti-PKA R2 antibody [4A3] used in IHC (Paraffin sections) (IHC-P). GTX83825
Anti-PKA R2 antibody [4A3] used in IHC (Paraffin sections) (IHC-P). GTX83825
Anti-PKA R2 antibody [4A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83825
Anti-PKA R2 antibody [4A3] used in IHC (Paraffin sections) (IHC-P). GTX83825
Anti-PKA R2 antibody [4A3] used in Western Blot (WB). GTX83825

Cat. No. GTX83825

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4A3

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($519)
See all PKA R2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human PRKAR2A (NP_004148) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase cAMP-dependent type II regulatory subunit alpha , PKR2 , PRKAR2

Cellular Localization

Cytoplasm,Cell membrane

Background

Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

Database

Research Area

DATA IMAGES

Anti-PKA R2 antibody [4A3] used in IHC (Paraffin sections) (IHC-P). GTX83825

GTX83825 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX83825 PKA R2 antibody [4A3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PKA R2 antibody [4A3] used in IHC (Paraffin sections) (IHC-P). GTX83825

GTX83825 IHC-P Image

IHC-P analysis of prostate tissue using GTX83825 PKA R2 antibody [4A3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PKA R2 antibody [4A3] used in IHC (Paraffin sections) (IHC-P). GTX83825

GTX83825 IHC-P Image

IHC-P analysis of liver tissue using GTX83825 PKA R2 antibody [4A3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PKA R2 antibody [4A3] used in IHC (Paraffin sections) (IHC-P). GTX83825

GTX83825 IHC-P Image

IHC-P analysis of liver carcinoma tissue using GTX83825 PKA R2 antibody [4A3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PKA R2 antibody [4A3] used in IHC (Paraffin sections) (IHC-P). GTX83825

GTX83825 IHC-P Image

IHC-P analysis of breast tissue using GTX83825 PKA R2 antibody [4A3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PKA R2 antibody [4A3] used in Western Blot (WB). GTX83825

GTX83825 WB Image

KO/KD

WB analysis of wild type and PKA R2 knock out HeLa cell lysate(10μg per lane) using GTX83825 PKA R2 antibody [4A3].
Dilution : 1:2000

Anti-PKA R2 antibody [4A3] used in IHC (Paraffin sections) (IHC-P). GTX83825

GTX83825 IHC-P Image

IHC-P analysis of kidney tissue using GTX83825 PKA R2 antibody [4A3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PKA R2 antibody [4A3] used in IHC (Paraffin sections) (IHC-P). GTX83825

GTX83825 IHC-P Image

IHC-P analysis of pancreas tissue using GTX83825 PKA R2 antibody [4A3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PKA R2 antibody [4A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83825

GTX83825 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with PKA R2 plasmid using GTX83825 PKA R2 antibody [4A3].

Anti-PKA R2 antibody [4A3] used in IHC (Paraffin sections) (IHC-P). GTX83825

GTX83825 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX83825 PKA R2 antibody [4A3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PKA R2 antibody [4A3] used in Western Blot (WB). GTX83825

GTX83825 WB Image

Overexpression

WB analysis of HEK293T cells transfected with PKA R2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83825 PKA R2 antibody [4A3].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519