Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKA R2 (phospho Ser99) antibody [GT1304] (GTX03216) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

WB analysis of C6 cells were treated by CIP(20uL/400ul) at 37ºC for 1 hour using GTX03216 PKA R2 (phospho Ser99) antibody [GT1304].
Dilution : 1:1000
Loading : 25μg per lane