Untreated (–) and treated (+) C6 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKA R2 (phospho Ser96) antibody (GTX130457) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKA R2 (phospho Ser99) antibody (GTX130457) diluted at 1:3000. The signal was developed with Trident ECL plus-Enhanced.