WB (peptide competition) analysis of 3T3-L1 cells using GTX25816 PKA beta (catalytic subunit) (phospho Ser338) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 1) eliminates the signal further verifying that the antibody is phospho-specific.

ChIP analysis of human 5A8 J-Lat T lymphocytes culture treated with 10 μg/mL PHA (phytohemaglutin) for 0, 2, and 24 hours using GTX25816 PKA beta (catalytic subunit) (phospho Ser338) antibody. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to -15kb upstream and exon-1 of the EGR1 gene.
IP reaction : 1.0ul antibody / 100ul sample