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PKC alpha antibody [133]

Cat. No. GTX11723

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 133

Isotype

 IgG2a

Application

 WB, IHC-Fr

Reactivity

 Mouse
Package
1 ml ($399)
See all PKC alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/10-1/500
IHC-Fr Neat-1/10
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Product Note

This antibody recognizes the sequence PQFVHPILQSAV at the C terminus of PKC alpha.

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Purification

Tissue Culture Supernatant

Conjugation

Unconjugated

RRID

AB_372727

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase C alpha , AAG6 , PKC-alpha , PKCA , PKCI+/- , PKCalpha , PRKACA

Cellular Localization

Cytoplasm , Cell membrane , Nucleus

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

Application Reference

Huang W et al. Vis Neurosci 2009; 26 (43226) : 443-52 Cell type-specific and light-dependent expression of Rab1 and Rab6 GTPases in mammalian retinas.
Application : WB, IHC-Fr
Reactivity : Mouse
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399