GeneTex

  • Country / Location Selection

United States (US)

PKC alpha antibody [C1C3]

Anti-PKC alpha antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111096
Anti-PKC alpha antibody [C1C3] used in Western Blot (WB). GTX111096
Anti-PKC alpha antibody [C1C3] used in Western Blot (WB). GTX111096

Cat. No. GTX111096

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PKC alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

Mouse brain , Jurkat

Predict Reactivity

Zebrafish, Rabbit, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PKC alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951408

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase C alpha , AAG6 , PKC-alpha , PKCA , PKCI+/- , PKCalpha , PRKACA

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PKC alpha antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111096

GTX111096 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using PKC alpha(GTX111096) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PKC alpha antibody [C1C3] used in Western Blot (WB). GTX111096

GTX111096 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111096 diluted at 1:2000

Anti-PKC alpha antibody [C1C3] used in Western Blot (WB). GTX111096

GTX111096 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha antibody [C1C3] (GTX111096) diluted at 1:5000.

REFERENCE

Application Reference

Kitayama T et al. Neurochem Int 2016; 101 : 120-131 Down-regulation of zinc transporter-1 in astrocytes induces neuropathic pain via the brain-derived neurotrophic factor - K+-Cl- co-transporter-2 signaling pathway in the mouse spinal cord.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for PKC alpha antibody [C1C3] (GTX111096). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169