PKC alpha antibody

Cat No. GTX130453

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-Fr
Reactivity	Human, Mouse, Rat, Zebrafish
Package	100 μl ($329), 25 μl ($169)

See all PKC alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-Fr	1:100-1:1000

Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

Jurkat , mouse brain , rat brain

Predict Reactivity

Rabbit, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PKC alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886274

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase C alpha , AAG6 , PKC-alpha , PKCA , PKCI+/- , PKCalpha , PRKACA

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq]

Database

Gene ID: 5578 PRKCA
UniProt: P17252 PRKCA

Research Area

DATA IMAGES

GTX130453 IHC-Fr Image

PKC alpha antibody detects PKC alpha protein in the rod bipolar cells by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: PKC alpha protein stained by PKC alpha antibody (GTX130453) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX130453 ICC/IF Image

PKC alpha antibody detects PKC alpha protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PKC alpha protein stained by PKC alpha antibody (GTX130453) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX130453 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha antibody (GTX130453) diluted at 1:2000.

GTX130453 IHC-Fr Image

PKC alpha antibody detects PKC alpha protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Red: PKC alpha protein stained by PKC alpha antibody (GTX130453) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX130453 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha antibody (GTX130453) diluted at 1:1000.

GTX130453 WB Image

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha antibody (GTX130453) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (GI: gastrointestinal)