PKC alpha (phospho Thr497) antibody [HL3605]

PKC proteins (0.01 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha (phospho Thr497) antibody [HL3605] (GTX641580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha (phospho Thr497) antibody [HL3605] (GTX641580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.