APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:3000 |
| 1:50-1:200 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Zebrafish, Dog, Chicken, Horse, Xenopus(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human PRKCB(Accession P05771), corresponding to amino acid residues around phosphorylated Ser661.
Purification
Purified by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
From serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein kinase C beta , PKC-beta , PKCB , PKCI(2) , PKCbeta , PRKCB1 , PRKCB2
Cellular Localization
Cytoplasm , Nucleus , Membrane
Background
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX04593 WB Image
WB analysis of mouse brain tissue lysate using GTX04593 PKC beta 1 (phospho Ser661) antibody. The lane on the left was treated with blocking peptide.
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GTX04593 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX04593 PKC beta 1 (phospho Ser661) antibody.
Antigen retrieval : Heat mediated citrate buffer.
Dilution : 1:200
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GTX04593 WB Image
WB analysis of P19 whole cell lysate with heat-shock treatment using GTX04593 PKC beta 1 (phospho Ser661) antibody. The lane on the left was treated with blocking peptide.
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GTX04593 WB Image
WB analysis of Raw264.7 whole cell lysate with LPS-treatment using GTX04593 PKC beta 1 (phospho Ser661) antibody. The lane on the left was treated with blocking peptide.
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GTX04593 ELISA Image
ELISA analysis of phospho- and non phosho- immunogen peptide using serial diluted GTX04593 PKC beta 1 (phospho Ser661) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1 μg/mL
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REFERENCE
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REVIEW
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