APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS pH 7.4 (without Mg²⁺ and Ca²⁺), 150 mM NaCl, 50% glycerol
Preservative
0.02% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide sequence around phosphorylation site of serine 661 (G-F-S(p)-Y-T) derived from Human PKCB.
Purification
Purified by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein kinase C beta , PKC-beta , PKCB , PKCI(2) , PKCbeta , PRKCB1 , PRKCB2
Cellular Localization
Cytoplasm , Nucleus , Membrane
Background
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX04594 WB Image
WB analysis of HeLa whole cell lysate with/without heat shock treatment using GTX04594 PKC beta 1 (phospho Ser661) antibody. The lane on the right is treated with the antigen-specific peptide.
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REFERENCE
There are currently no references for PKC beta 1 (phospho Ser661) antibody (GTX04594). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PKC beta 1 (phospho Ser661) antibody (GTX04594). Be the first to share your experience with this product.
