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PKC beta 2 antibody [ST48-06]

Recombinant antibody
Anti-PKC beta 2 antibody [ST48-06] used in Western Blot (WB). GTX01037
Anti-PKC beta 2 antibody [ST48-06] used in IHC (Paraffin sections) (IHC-P). GTX01037
Anti-PKC beta 2 antibody [ST48-06] used in IHC (Paraffin sections) (IHC-P). GTX01037
Anti-PKC beta 2 antibody [ST48-06] used in Flow cytometry (FACS). GTX01037
Anti-PKC beta 2 antibody [ST48-06] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01037
Anti-PKC beta 2 antibody [ST48-06] used in IHC (Paraffin sections) (IHC-P). GTX01037

Cat No. GTX01037

Host

Rabbit

Clonality

Monoclonal

Clone Name

ST48-06

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse
Package
100 μl ($369),
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase C beta,PKCB,PKCbeta,PRKCB,PRKCB1,PRKCB2,PKC beta

Cellular Localization

Cytoplasm,Nucleus,Membrane

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PKC beta 2 antibody [ST48-06] used in Western Blot (WB). GTX01037

GTX01037 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC beta 2 antibody [ST48-06] (GTX01037) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PKC beta 2 antibody [ST48-06] used in IHC (Paraffin sections) (IHC-P). GTX01037

GTX01037 IHC-P Image

IHC-P analysis of mouse spleen tissue section using GTX01037 PKC beta 2 antibody [ST48-06].

Anti-PKC beta 2 antibody [ST48-06] used in IHC (Paraffin sections) (IHC-P). GTX01037

GTX01037 IHC-P Image

IHC-P analysis of mouse brain tissue section using GTX01037 PKC beta 2 antibody [ST48-06].

Anti-PKC beta 2 antibody [ST48-06] used in Flow cytometry (FACS). GTX01037

GTX01037 FACS Image

FACS analysis of HeLa cells using GTX01037 PKC beta 2 antibody [ST48-06].
Blue : primary antibody
Red : unlabelled control
Dilution : 1:50

Anti-PKC beta 2 antibody [ST48-06] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01037

GTX01037 ICC/IF Image

ICC/IF analysis of PFA-fixed SH-SY-5Y cells using GTX01037 PKC beta 2 antibody [ST48-06].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-PKC beta 2 antibody [ST48-06] used in IHC (Paraffin sections) (IHC-P). GTX01037

GTX01037 IHC-P Image

IHC-P analysis of human spleen tissue section using GTX01037 PKC beta 2 antibody [ST48-06].

REFERENCE

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REVIEW

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