APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:8,000 |
| 1:1,000 |
Not tested in other applications.
Calculated MW
Positive Control
rat brain
Product Note
In immunoblotting, the product reacts with PKC β2 (80 kDa) using rat brain extract.
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
synthetic peptide corresponding to the C-terminal region of PKC β2 (660-673).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein kinase C, beta , Pkcb , Prkcb1
Cellular Localization
Cytoplasm,Nucleus
Background
Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-564', 'Ser-649' and 'Ser-657', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
Database
Research Area
REFERENCE
There are currently no references for PKC beta 2 antibody (GTX39622). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PKC beta 2 antibody (GTX39622). Be the first to share your experience with this product.
