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PKC beta 2 (phospho Thr641) antibody

Anti-PKC beta 2 (phospho Thr641) antibody used in Western Blot (WB). GTX50682
Anti-PKC beta 2 (phospho Thr641) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50682
Anti-PKC beta 2 (phospho Thr641) antibody used in IHC (Paraffin sections) (IHC-P). GTX50682

Cat No. GTX50682

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 641 (E-L-T(p)-P-T) derived from human PKC beta 2.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase C beta , PKC-beta , PKCB , PKCI(2) , PKCbeta , PRKCB1 , PRKCB2

Cellular Localization

Cytoplasm,Nucleus,Membrane

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PKC beta 2 (phospho Thr641) antibody used in Western Blot (WB). GTX50682

GTX50682 WB Image

WB analysis of extracts from JK cells untreated or treated with PMA using GTX50682 PKC beta 2 (phospho Thr641) antibody.

Anti-PKC beta 2 (phospho Thr641) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50682

GTX50682 ICC/IF Image

ICC/IF analysis of methanol-fixed MCF-7 cells using GTX50682 PKC beta 2 (phospho Thr641) antibody.

Anti-PKC beta 2 (phospho Thr641) antibody used in IHC (Paraffin sections) (IHC-P). GTX50682

GTX50682 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX50682 PKC beta 2 (phospho Thr641) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf