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PKC delta antibody

Orthogonal
Anti-PKC delta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50605
Anti-PKC delta antibody used in Western Blot (WB). GTX50605
Anti-PKC delta antibody used in IHC (Paraffin sections) (IHC-P). GTX50605

Cat. No. GTX50605

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all PKC delta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa. 643~647 (R-L-S-Y-S) derived from human PKC delta.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase C delta , ALPS3 , CVID9 , MAY1 , PKCD , nPKC-delta

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-PKC delta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50605

GTX50605 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50605 PKC delta antibody.

Anti-PKC delta antibody used in Western Blot (WB). GTX50605

GTX50605 WB Image

WB analysis of extracts from PC12 cells using GTX50605 PKC delta antibody.

Anti-PKC delta antibody used in IHC (Paraffin sections) (IHC-P). GTX50605

GTX50605 IHC-P Image

Orthogonal

IHC-P analysis of human breast carcinoma tissue using GTX50605 PKC delta antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

Application Reference

Dharmarajan S et al. Diabetologia 2023; 66 (11) : 2170-2185 Retinal inflammation in murine models of type 1 and type 2 diabetes with diabetic retinopathy.
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399