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PKC delta (phospho Ser645) antibody

Anti-PKC delta (phospho Ser645) antibody used in Western Blot (WB). GTX50314
Anti-PKC delta (phospho Ser645) antibody used in IHC (Paraffin sections) (IHC-P). GTX50314

Cat No. GTX50314

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($319)
See all PKC delta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 645 (R-L-S(p)-Y-S) derived from human PKC delta.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase C delta , ALPS3 , CVID9 , MAY1 , PKCD , nPKC-delta

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-PKC delta (phospho Ser645) antibody used in Western Blot (WB). GTX50314

GTX50314 WB Image

WB analysis of extracts from PC12 cells untreated or treated with PMA using GTX50314 PKC delta (phospho Ser645) antibody.

Anti-PKC delta (phospho Ser645) antibody used in IHC (Paraffin sections) (IHC-P). GTX50314

GTX50314 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50314 PKC delta (phospho Ser645) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319