WB (peptide competition) analysis of K562 cells stimulated with PMA using GTX25658 PKC delta (phospho Ser664) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphoserine-containing peptide (Lane 3), the phosphopeptide immunogen (Lane 4), or the phosphopeptides corresponding to other PKC isoforms (Lane 5-12)control.The data show that the peptide corresponding to PKCdelta (pS664) blocks the antibody signal. The peptide corresponding to PKCalpha (pS657) partially blocks the antibody signal, while those for PKCbetaII (pS660), and rat PKCdelta (pS662) block the antibody signal more thoroughly, indicating their respective reactivity with this antibody. The membrane treated with phosphatase (Lane 13) eliminates the signal further verifying that the antibody is phospho-specific.

IHC-P analysis of human breast carcinoma tissue using GTX25658 PKC delta (phospho Ser664) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20