PKC delta (phospho Ser664) antibody

Cat. No. GTX25658

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Rat
Package	50 μl ($399)

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Datasheet
Document

View product citations for antibody GTX25658 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
IHC-P	1:10-1:50

Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Product Note

This antibody has been shown to cross-react with rat PKC alpha [pS657] (75% homologous) and PKC beta II [pS66] (67% homologous) shown by peptide competition experiments. Mouse PKC delta [pS662] (83% homologous) has not been tested, but is expected to react.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PKCd that contains serine 664.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase C delta , ALPS3 , CVID9 , MAY1 , PKCD , nPKC-delta

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome. [provided by RefSeq, Aug 2017]

Database

Gene ID: 5580 PRKCD
UniProt: Q05655 PRKCD

Research Area

DATA IMAGES

GTX25658 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX25658 PKC delta (phospho Ser664) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

GTX25658 WB Image

WB (peptide competition) analysis of K562 cells stimulated with PMA using GTX25658 PKC delta (phospho Ser664) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphoserine-containing peptide (Lane 3), the phosphopeptide immunogen (Lane 4), or the phosphopeptides corresponding to other PKC isoforms (Lane 5-12)control.The data show that the peptide corresponding to PKCdelta (pS664) blocks the antibody signal. The peptide corresponding to PKCalpha (pS657) partially blocks the antibody signal, while those for PKCbetaII (pS660), and rat PKCdelta (pS662) block the antibody signal more thoroughly, indicating their respective reactivity with this antibody. The membrane treated with phosphatase (Lane 13) eliminates the signal further verifying that the antibody is phospho-specific.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

50 μl

$ 399

Qty