APPLICATION
Application Note
Calculated MW
Positive Control
293T , 293T (125 ng PMA treatment for 30min and 500 ng PMA treatment for 30 min) , MCF-7 treated with LPS
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic phosphopeptide derived from human PKC δ around the phosphorylation site of Threonine 507.
Purification
Purified by antigen-affinity chromatography
From serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Protein Kinase C Delta , Alps3 , Cvid9 , May1 , Pkcd , Npkc-Delta , Prkcd
Background
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
Research Area
DATA IMAGES
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GTX20305 WB Image
Untreated (–) and treated (+) 293T whole cell extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC delta (phospho Thr507) antibody (GTX20305) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX20305 WB Image
WB analysis of various samples using GTX20305 PKC delta (phospho Thr507) antibody.
Lane1 : MCF-7 whole cell lysate
Lane2 : MCF-7 treated with LPS(100ng/ml) for 5 minutes whole cell lysate
Lane3 : MCF-7 treated with LPS(100ng/ml) for 30 minutes whole cell lysate
Dilution : 1:500
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REFERENCE
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REVIEW
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