Untreated (–) and treated (+) 293T whole cell extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC delta (phospho Thr507) antibody (GTX20305) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

WB analysis of various samples using GTX20305 PKC delta (phospho Thr507) antibody.
Lane1 : MCF-7 whole cell lysate
Lane2 : MCF-7 treated with LPS(100ng/ml) for 5 minutes whole cell lysate
Lane3 : MCF-7 treated with LPS(100ng/ml) for 30 minutes whole cell lysate
Dilution : 1:500