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PKC delta (phospho Thr507) antibody

Anti-PKC delta (phospho Thr507) antibody used in IHC (Paraffin sections) (IHC-P). GTX32244
Anti-PKC delta (phospho Thr507) antibody used in Western Blot (WB). GTX32244

Cat No. GTX32244

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

78 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of PKC delta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Kinase C Delta,Alps3,Cvid9,May1,Pkcd,Npkc-Delta,Prkcd

Cellular Localization

Cell membrane,Cytoplasm,Nucleus,Perinuclear region

Background

The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES
Anti-PKC delta (phospho Thr507) antibody used in IHC (Paraffin sections) (IHC-P). GTX32244

GTX32244 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32244 PKC delta (phospho Thr507) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-PKC delta (phospho Thr507) antibody used in Western Blot (WB). GTX32244

GTX32244 WB Image

WB analysis of H2O2-treated HeLa (A), H2O2-treated A549 (B), H2O2-treated Raw264.7 (C), H2O2-treated PC12 (D) whole cell lysates using GTX32244 PKC delta (phospho Thr507) antibody.