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PKC epsilon (phospho Ser729) antibody

Orthogonal
Anti-PKC epsilon (phospho Ser729) antibody used in Western Blot (WB). GTX133937
Anti-PKC epsilon (phospho Ser729) antibody used in IHC (Paraffin sections) (IHC-P). GTX133937
Anti-PKC epsilon (phospho Ser729) antibody used in Western Blot (WB). GTX133937
Anti-PKC epsilon (phospho Ser729) antibody used in IHC (Paraffin sections) (IHC-P). GTX133937

Cat. No. GTX133937

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PKC epsilon products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Predict Reactivity

Rabbit, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser729 of human PKC epsilon. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887141

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase C epsilon , PKCE , nPKC-epsilon

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PKC epsilon (phospho Ser729) antibody used in Western Blot (WB). GTX133937

GTX133937 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC epsilon (phospho Ser729) antibody (GTX133937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PKC epsilon (phospho Ser729) antibody used in IHC (Paraffin sections) (IHC-P). GTX133937

GTX133937 IHC-P Image

PKC epsilon (phospho Ser729) antibody detects PKC epsilon (phospho Ser729) protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
PKC epsilon (phospho Ser729) stained by PKC epsilon (phospho Ser729) antibody (GTX133937) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PKC epsilon (phospho Ser729) antibody used in Western Blot (WB). GTX133937

GTX133937 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC epsilon (phospho Ser729) antibody (GTX133937) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PKC epsilon (phospho Ser729) antibody used in IHC (Paraffin sections) (IHC-P). GTX133937

GTX133937 IHC-P Image

PKC epsilon (phospho Ser729) antibody detects PKC epsilon (phospho Ser729) protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
PKC epsilon (phospho Ser729) stained by PKC epsilon (phospho Ser729) antibody (GTX133937) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Lee PC et al. Am J Cancer Res 2023; 13 (10) : 4693-4707 Development of triazole-based PKC-inhibitors to overcome resistance to EGFR inhibitors in EGFR-mutant lung cancers.
Min Liu et al. Hum Cell 2022; 35 (2) : 678-693
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169