GeneTex

  • Country / Location Selection

United States (US)

PKC epsilon (phospho Ser729) antibody

Orthogonal
Anti-PKC epsilon (phospho Ser729) antibody used in Western Blot (WB). GTX25811

Cat. No. GTX25811

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
50 μl ($399)
See all PKC epsilon products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Product Note

This antibody may partially cross react with beta 2 [pS66] as determined by peptide competition experiments.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PKCe that contains serine 729. The sequence is conserved in mouse and rat.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase C epsilon , PKCE , nPKC-epsilon

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton,Cell membrane,Nucleus

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PKC epsilon (phospho Ser729) antibody used in Western Blot (WB). GTX25811

GTX25811 WB Image

Orthogonal

WB analysis of K562 cells stimulated with PMA using GTX25811 PKC epsilon (phospho Ser729) antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal verifying that the antibody is phospho-specific.

REFERENCE

There are currently no references for PKC epsilon (phospho Ser729) antibody (GTX25811). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for PKC epsilon (phospho Ser729) antibody (GTX25811). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399