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PKC gamma antibody [HL2226]

Recombinant antibody Orthogonal
Anti-PKC gamma antibody [HL2226] used in Western Blot (WB). GTX638271
Anti-PKC gamma antibody [HL2226] used in IHC (Paraffin sections) (IHC-P). GTX638271
Anti-PKC gamma antibody [HL2226] used in IHC (Paraffin sections) (IHC-P). GTX638271
Anti-PKC gamma antibody [HL2226] used in Western Blot (WB). GTX638271
Anti-PKC gamma antibody [HL2226] used in Western Blot (WB). GTX638271

Cat. No. GTX638271

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2226

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PKC gamma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Product Note

This antibody is specific for human PKC gamma protein, and it does not cross react with human PKC alpha, PKC beta 1, PKC beta 2, PKC delta, PKC epsilon, PKC zeta, PKC eta, and PKC theta protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragmemt of human PKC gamma.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase C gamma , PKC-gamma , PKCC , PKCG , PKCI(3) , PKCgamma , SCA14

Cellular Localization

Cytoplasm,Cytoplasm, perinuclear region,Cell membrane,Synapse, synaptosome,Cell projection, dendrite

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-PKC gamma antibody [HL2226] used in Western Blot (WB). GTX638271

GTX638271 WB Image

Orthogonal

PKC proteins (10 ng) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody [HL2226] (GTX638271) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PKC gamma antibody [HL2226] used in IHC (Paraffin sections) (IHC-P). GTX638271

GTX638271 IHC-P Image

PKC gamma antibody [HL2226] detects PKC gamma protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
PKC gamma stained by PKC gamma antibody [HL2226] (GTX638271) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PKC gamma antibody [HL2226] used in IHC (Paraffin sections) (IHC-P). GTX638271

GTX638271 IHC-P Image

PKC gamma antibody [HL2226] detects PKC gamma protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
PKC gamma stained by PKC gamma antibody [HL2226] (GTX638271) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PKC gamma antibody [HL2226] used in Western Blot (WB). GTX638271

GTX638271 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody [HL2226] (GTX638271) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PKC gamma antibody [HL2226] used in Western Blot (WB). GTX638271

GTX638271 WB Image

Human brain (5 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody [HL2226] (GTX638271) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations