PKC gamma antibody [HL2227]

PKC gamma antibody [HL2227] detects PKC gamma protein at cytoplasm by immunofluorescent analysis.
Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PKC gamma stained by PKC gamma antibody [HL2227] (GTX638272) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody [HL2227] (GTX638272) diluted at 1:5000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PKC gamma antibody [HL2227] detects PKC gamma protein at neurite by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PKC gamma stained by PKC gamma antibody [HL2227] (GTX638272) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

PKC proteins (10 ng) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody [HL2227] (GTX638272) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.