*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Molt-4 , mouse brain , rat brain
Rabbit, Bovine(>80% identity)
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.55mg/ml(Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the N-terminus region of human PKC gamma. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
MGC57564 antibody, PKC-gamma antibody, PKCC antibody, PKCG antibody, SCA14 antibody, PRKCG antibody, protein kinase C gamma type antibody, "protein kinase C, gamma antibody"
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). [provided by RefSeq]
GTX102539 WB Image
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody (GTX102539) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GTX102539 IHC-Fr Image
PKC gamma antibody detects PKC gamma protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: PKC gamma protein stained by PKC gamma antibody (GTX102539) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
GTX102539 WB Image
Sample(30 ug whole cell lysate)
7.5% SDS PAGE
GTX102539 diluted at 1:500