GeneTex
United States (US)

PKC gamma antibody

GTX107639_WB_19011506_875.jpg
GTX107639_39981_20170608_IHC-P_M_19011506_711.jpg
GTX107639_39981_20170922_WB_M_R_19011506_955.jpg
GTX107639_39981_20160201_IHC-P_R_19011506_714.jpg
GTX107639_39981_IHC_19011506_164.jpg
GTX107639_39981_20150402_IHC_M_19011507_341.jpg

Cat No. GTX107639

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

293T , mouse brain , rat brain

Predict Reactivity

Rabbit, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKC gamma. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Kinase C Gamma,Pkc-Gamma,Pkcc,Pkcg,Pkci(3),Pkcgamma,Sca14,Prkcg

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107639_WB_19011506_875.jpg

GTX107639 WB Image

Sample(30 ug whole cell lysate)
A:293T
7.5% SDS PAGE
GTX107639 diluted at 1:2000

GTX107639_39981_20170608_IHC-P_M_19011506_711.jpg

GTX107639 IHC-Fr Image

PKC gamma antibody detects PKC gamma protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: PKC gamma protein stained by PKC gamma antibody (GTX107639) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX107639_39981_20170922_WB_M_R_19011506_955.jpg

GTX107639 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody (GTX107639) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107639_39981_20160201_IHC-P_R_19011506_714.jpg

GTX107639 IHC-P Image

PKC gamma antibody detects PKC gamma protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
PKC gamma antibody (GTX107639) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107639_39981_IHC_19011506_164.jpg

GTX107639 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hun7 xenograft, using PKC gamma(GTX107639) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107639_39981_20150402_IHC_M_19011507_341.jpg

GTX107639 IHC-P Image

PKC gamma antibody detects PKC gamma protein at cytoplasm on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
PKC gamma antibody (GTX107639) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169