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PKC gamma antibody

Anti-PKC gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX107639
Anti-PKC gamma antibody used in IHC (Frozen sections) (IHC-Fr). GTX107639
Anti-PKC gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX107639
Anti-PKC gamma antibody used in Western Blot (WB). GTX107639
Anti-PKC gamma antibody used in Western Blot (WB). GTX107639
Anti-PKC gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX107639
Anti-PKC gamma antibody used in Western Blot (WB). GTX107639
Anti-PKC gamma antibody used in Western Blot (WB). GTX107639

Cat. No. GTX107639

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PKC gamma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Rabbit, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKC gamma. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951418

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase C gamma , PKC-gamma , PKCC , PKCG , PKCI(3) , PKCgamma , SCA14

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PKC gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX107639

GTX107639 IHC-P Image

PKC gamma antibody detects PKC gamma protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
PKC gamma antibody (GTX107639) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PKC gamma antibody used in IHC (Frozen sections) (IHC-Fr). GTX107639

GTX107639 IHC-Fr Image

PKC gamma antibody detects PKC gamma protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: PKC gamma protein stained by PKC gamma antibody (GTX107639) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-PKC gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX107639

GTX107639 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hun7 xenograft, using PKC gamma(GTX107639) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PKC gamma antibody used in Western Blot (WB). GTX107639

GTX107639 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody (GTX107639) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PKC gamma antibody used in Western Blot (WB). GTX107639

GTX107639 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody (GTX107639) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PKC gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX107639

GTX107639 IHC-P Image

PKC gamma antibody detects PKC gamma protein at cytoplasm on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
PKC gamma antibody (GTX107639) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PKC gamma antibody used in Western Blot (WB). GTX107639

GTX107639 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody (GTX107639) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PKC gamma antibody used in Western Blot (WB). GTX107639

GTX107639 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody (GTX107639) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Zhang X et al. Mol Pain 2021; 17 : 17448069211061973 The PKCγ neurons in anterior cingulate cortex contribute to the development of neuropathic allodynia and pain-related emotion.
Reactivity : Mouse
Liu P et al. Mol Pain 2021; 17 : 1744806921992620 Spinal GABAergic neurons are under feed-forward inhibitory control driven by Aδ and C fibers in Gad2 td-Tomato mice.
Application : IHC-Fr
Reactivity : Mouse
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169