APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 |
| 1:10-1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 1mg/ml BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PKC-gamma that contains threonine 514
Purification
Purified IgG
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein kinase C gamma , PKC-gamma , PKCC , PKCG , PKCI(3) , PKCgamma , SCA14
Cellular Localization
Cytoplasm,Cell membrane,Cell projection, dendrite
Background
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]
Database
Research Area
DATA IMAGES
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GTX25778 IHC-P Image
IHC-P analysis of human cerebellum tissue using GTX25778 PKC gamma (phospho Thr514) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20
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GTX25778 WB Image
WB analysis of whole cell extracts (30 μg lysate) of Jurkat (Lane 1) and A549 (Lane 2) using GTX25778 PKC gamma (phospho Thr514) antibody.
Dilution : 1:500
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GTX25778 WB Image
WB (peptide competition) analysis of HeLa cells stimulated with PMA using GTX25778 PKC gamma (phospho Thr514) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphothreonine containing peptide (Lane 3), the phosphopeptide immunogen (Lane 4), or, the phosphopeptide corresponding to the immunogen from other PKC isoforms (Lane 5-9). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 11) eliminates the signal further verifying that the antibody is phospho-specific.
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REFERENCE
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REVIEW
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