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PKC gamma (phospho Thr655) antibody

Anti-PKC gamma (phospho Thr655) antibody used in IHC (Paraffin sections) (IHC-P). GTX25796
Anti-PKC gamma (phospho Thr655) antibody used in IHC (Paraffin sections) (IHC-P). GTX25796
Anti-PKC gamma (phospho Thr655) antibody used in Western Blot (WB). GTX25796
Anti-PKC gamma (phospho Thr655) antibody used in Western Blot (WB). GTX25796

Cat No. GTX25796

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, FACS

Reactivity

Human, Mouse
Package
50 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:250
IHC-P 1:10-1:50
FACS 1:20
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PKC-gamma that contains threonine 655

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase C gamma , PKC-gamma , PKCC , PKCG , PKCI(3) , PKCgamma , SCA14

Cellular Localization

Cytoplasm,Cell membrane,Cell projection, dendrite

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-PKC gamma (phospho Thr655) antibody used in IHC (Paraffin sections) (IHC-P). GTX25796

GTX25796 IHC-P Image

IHC-P analysis of human cerebellum tissue using GTX25796 PKC gamma (phospho Thr655) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-PKC gamma (phospho Thr655) antibody used in IHC (Paraffin sections) (IHC-P). GTX25796

GTX25796 IHC-P Image

IHC-P analysis of human cerebellum tissue using GTX25796 PKC gamma (phospho Thr655) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-PKC gamma (phospho Thr655) antibody used in Western Blot (WB). GTX25796

GTX25796 WB Image

WB analysis of whole cell extracts (30 μg lysate) of HCT 116 (Lane 1), treated for 20 minutes with 200 mM of PMA (Lane 2), and A549 (Lane 3) treated for 20 minutes with 200 mM of PMA (Lane 4) using GTX25796 PKC gamma (phospho Thr655) antibody.
Dilution : 1:500

Anti-PKC gamma (phospho Thr655) antibody used in Western Blot (WB). GTX25796

GTX25796 WB Image

WB analysis of samples using GTX25796 PKC gamma (phospho Thr655) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 12) eliminates the signal further verifying that the antibody is phospho-specific.
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379