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PKC gamma (phospho Thr674) antibody

Anti-PKC gamma (phospho Thr674) antibody used in IHC (Paraffin sections) (IHC-P). GTX25797
Anti-PKC gamma (phospho Thr674) antibody used in IHC (Paraffin sections) (IHC-P). GTX25797

Cat No. GTX25797

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
50 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P 1:10-1:50
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Product Note

The peptide competition data (see Figure) suggest that this antibody will cross-react with PKC alpha [pS657] (69% homologous), beta 1 [pS661] (54%) and PKC eta [pS674] (38%).

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 1mg/ml BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PKCg that contains threonine 674. The sequence is conserved in mouse and rat.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380692

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase C gamma , PKC-gamma , PKCC , PKCG , PKCI(3) , PKCgamma , SCA14

Cellular Localization

Cytoplasm,Cell membrane,Cell projection, dendrite

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-PKC gamma (phospho Thr674) antibody used in IHC (Paraffin sections) (IHC-P). GTX25797

GTX25797 IHC-P Image

IHC-P analysis of human cerebellum tissue using GTX25797 PKC gamma (phospho Thr674) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-PKC gamma (phospho Thr674) antibody used in IHC (Paraffin sections) (IHC-P). GTX25797

GTX25797 IHC-P Image

IHC-P analysis of human cerebellum tissue using GTX25797 PKC gamma (phospho Thr674) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379