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PKC mu antibody

Comparison
Anti-PKC mu antibody used in Western Blot (WB). GTX133778
Anti-PKC mu antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133778

Cat. No. GTX133778

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PKC mu products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

NIH3T3

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse PKC mu. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887106

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase D1 , PKD , PKD1 , Pkcm , Prkcm

Background

May be the GTPase, regulating ATP sulfurylase activity.

Database

Research Area

DATA IMAGES

Anti-PKC mu antibody used in Western Blot (WB). GTX133778

GTX133778 WB Image

Comparison

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with PKC mu antibody (GTX133778) diluted at 1:1000 and competitor's antibody (sc-639) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-PKC mu antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133778

GTX133778 ICC/IF Image

PKC mu antibody detects PKC mu protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PKC mu protein stained by PKC mu antibody (GTX133778) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169