GeneTex
United States (US)

PKC mu (phospho Ser738) antibody

GTX38607_20190129_WB_19012901_859.jpg
GTX38607_20190129_ICCIF_19012901_663.jpg

Cat No. GTX38607

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

102 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS (without Mg2+ and Ca2+) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human PKD/PKC mu around the phosphorylation site of serine 738 (E -K-S(p)-F-R).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Kinase D1,Chded,Pkc-Mu,Pkcm,Pkd,Prkcm,Prkd1

Cellular Localization

Cell membrane,Cytoplasm,Golgi apparatus,Trans-Golgi network

Background

The protein encoded by this gene is a serine/threonine protein kinase involved in many cellular processes, including Golgi body membrane integrity and transport, cell migration and differentiation, MAPK8/JNK1 and Ras pathway signaling, MAPK1/3 (ERK1/2) pathway signaling, cell survival, and regulation of cell shape and adhesion. [provided by RefSeq, Jan 2017]

Database

Research Area

DATA IMAGES
GTX38607_20190129_WB_19012901_859.jpg

GTX38607 WB Image

WB analysis of extracts of A431 cells untreated or treated with EGF (200ng/ml, 10min) using PKC mu antibody (Lane 1 and 2) and GTX38607 PKC mu (phospho Ser738) antibody(Lane 3 and 4).

GTX38607_20190129_ICCIF_19012901_663.jpg

GTX38607 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX38607 PKC mu (phospho Ser738) antibody.
Red : Primary antibody

Package List Price ($)
$ 219