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PKC mu (phospho Ser910) antibody

Orthogonal
Anti-PKC mu (phospho Ser910) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50160
Anti-PKC mu (phospho Ser910) antibody used in Western Blot (WB). GTX50160

Cat. No. GTX50160

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all PKC mu products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 910 (R-V-S(p)-I-L) derived from human PKC mu.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase D1 , CHDED , PKC-MU , PKCM , PKD , PRKCM

Cellular Localization

Cytoplasm,Cell membrane

Background

The protein encoded by this gene is a serine/threonine protein kinase involved in many cellular processes, including Golgi body membrane integrity and transport, cell migration and differentiation, MAPK8/JNK1 and Ras pathway signaling, MAPK1/3 (ERK1/2) pathway signaling, cell survival, and regulation of cell shape and adhesion. [provided by RefSeq, Jan 2017]

Database

Research Area

DATA IMAGES

Anti-PKC mu (phospho Ser910) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50160

GTX50160 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50160 PKC mu (phospho Ser910) antibody.

Anti-PKC mu (phospho Ser910) antibody used in Western Blot (WB). GTX50160

GTX50160 WB Image

Orthogonal

WB analysis of extracts from EGF-treated 293 and PMA-treated HUVEC cells using GTX50160 PKC mu (phospho Ser910) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399