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PKC nu antibody

Anti-PKC nu antibody used in Western Blot (WB). GTX25285
Anti-PKC nu antibody used in IHC (Paraffin sections) (IHC-P). GTX25285

Cat. No. GTX25285

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
400 μl ($399)
See all PKC nu products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

HL60 cell lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 352-384 amino acids from human PKC-nu.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase D3 , EPK2 , PKC-NU , PKD3 , PRKCN , nPKC-NU

Cellular Localization

Cytoplasm,Membrane

Background

This gene belongs to the multigene protein kinase D family of serine/threonine kinases, which bind diacylglycerol and phorbol esters. Members of this family are characterized by an N-terminal regulatory domain comprised of a tandem repeat of cysteine-rich zinc-finger motifs and a pleckstrin domain. The C-terminal region contains the catalytic domain and is distantly related to calcium-regulated kinases. Catalytic activity of this enzyme promotes its nuclear localization. This protein has been implicated in a variety of functions including negative regulation of human airway epithelial barrier formation, growth regulation of breast and prostate cancer cells, and vesicle trafficking. [provided by RefSeq, Jan 2015]

Database

Research Area

DATA IMAGES

Anti-PKC nu antibody used in Western Blot (WB). GTX25285

GTX25285 WB Image

WB analysis of HL-60 cells stimulated with PMA (lane A) and mouse brain tissue lysate (lane B) using GTX25285 PKC nu antibody.

Anti-PKC nu antibody used in IHC (Paraffin sections) (IHC-P). GTX25285

GTX25285 IHC-P Image

IHC-P analysis of human breast carcinoma using GTX25285 PKC nu antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399