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PKC (pan) (zeta phospho Thr410) antibody [HL1278]

Recombinant antibody Orthogonal
Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] used in Western Blot (WB). GTX636680
Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] used in Western Blot (WB). GTX636680
Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] used in Western Blot (WB). GTX636680
Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] used in Western Blot (WB). GTX636680
Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] used in Western Blot (WB). GTX636680

Cat. No. GTX636680

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1278

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all PKC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr410 of human PKC zeta. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase C alpha , protein kinase C beta , protein kinase C delta , protein kinase C epsilon , protein kinase C gamma , protein kinase C eta , protein kinase C iota , protein kinase C theta , protein kinase C zeta , AAG6 , PKC-alpha , PKCA , PKCI+/- , PKCalpha , PRKACA , PKC-beta , PKCB , PKCI(2) , PKCbeta , PRKCB1 , PRKCB2 , ALPS3 , CVID9 , MAY1 , PKCD , nPKC-delta , PKCE , nPKC-epsilon , PKC-gamma , PKCC , PKCG , PKCI(3) , PKCgamma , SCA14 , PKC-L , PKCL , PRKCL , nPKC-eta , DXS1179E , PKCI , nPKC-iota , PRKCT , nPKC-theta , PKC-ZETA , PKC2

Cellular Localization

Cytoplasm , Cytoplasm, cytoskeleton , Cell membrane , Cytoplasm, perinuclear region , Nucleus , Synapse, synaptosome , Cell projection, dendrite , Membrane , Endosome , Cell junction

Database

Research Area

DATA IMAGES

Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] used in Western Blot (WB). GTX636680

GTX636680 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1278] (GTX636680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] used in Western Blot (WB). GTX636680

GTX636680 WB Image

Orthogonal

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1278] (GTX636680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] used in Western Blot (WB). GTX636680

GTX636680 WB Image

Orthogonal

PKC proteins were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1278] (GTX636680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] used in Western Blot (WB). GTX636680

GTX636680 WB Image

Drosophila tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1278] (GTX636680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] used in Western Blot (WB). GTX636680

GTX636680 WB Image

Orthogonal

Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1278] (GTX636680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations