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PKC theta antibody

Anti-PKC theta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50606
Anti-PKC theta antibody used in IHC (Paraffin sections) (IHC-P). GTX50606
Anti-PKC theta antibody used in Western Blot (WB). GTX50606

Cat. No. GTX50606

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all PKC theta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa. 674~678 (R-L-S-F-A) derived from human PKC theta.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase C theta , PRKCT , nPKC-theta

Cellular Localization

Cytoplasm

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PKC theta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50606

GTX50606 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50606 PKC theta antibody.

Anti-PKC theta antibody used in IHC (Paraffin sections) (IHC-P). GTX50606

GTX50606 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX50606 PKC theta antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-PKC theta antibody used in Western Blot (WB). GTX50606

GTX50606 WB Image

WB analysis of extracts from PC12 cells using GTX50606 PKC theta antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399