GeneTex
United States (US)

PKC theta (phospho Ser676) antibody

GTX32246_20190131_ICCIF_19013101_734.jpg
GTX32246_20190131_WB_19013101_435.jpg
GTX32246_20190131_IHC-P_19013101_361.jpg

Cat No. GTX32246

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

82 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of PKC theta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Kinase C Theta,Prkct,Npkc-Theta,Prkcq

Cellular Localization

Cell membrane,Cytoplasm

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX32246_20190131_ICCIF_19013101_734.jpg

GTX32246 ICC/IF Image

ICC/IF analysis of formalin-fixed HeLa cells using GTX32246 PKC theta (phospho Ser676) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

GTX32246_20190131_WB_19013101_435.jpg

GTX32246 WB Image

WB analysis of UV-treated HeLa (A), UV-treated SP2/0 (B), UV-treated PC12 (C) whole cell lysates using GTX32246 PKC theta (phospho Ser676) antibody.

GTX32246_20190131_IHC-P_19013101_361.jpg

GTX32246 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32246 PKC theta (phospho Ser676) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)