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PKC theta (phospho Ser695) antibody

Anti-PKC theta (phospho Ser695) antibody used in Western Blot (WB). GTX50223
Anti-PKC theta (phospho Ser695) antibody used in IHC (Paraffin sections) (IHC-P). GTX50223

Cat No. GTX50223

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 695 (N-F-S(p)-F-M) derived from human PKC theta.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase C theta , PRKCT , nPKC-theta

Cellular Localization

Cytoplasm

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PKC theta (phospho Ser695) antibody used in Western Blot (WB). GTX50223

GTX50223 WB Image

WB analysis of extracts from 293 cells untreated or treated with PMA using GTX50223 PKC theta (phospho Ser695) antibody.

Anti-PKC theta (phospho Ser695) antibody used in IHC (Paraffin sections) (IHC-P). GTX50223

GTX50223 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX50223 PKC theta (phospho Ser695) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf