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PKC zeta antibody

Anti-PKC zeta antibody used in IHC (Paraffin sections) (IHC-P). GTX66624
Anti-PKC zeta antibody used in Western Blot (WB). GTX66624

Cat No. GTX66624

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids 380-420 of Human PKC ζ.

Purification

Purified by antigen-affinity chromatography
From serum

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase C zeta , PKC-ZETA , PKC2

Cellular Localization

Cytoplasm,Endosome,Cell junction,Membrane

Background

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PKC zeta antibody used in IHC (Paraffin sections) (IHC-P). GTX66624

GTX66624 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX66624 PKC zeta antibody.
Dilution : 1:100

Anti-PKC zeta antibody used in Western Blot (WB). GTX66624

GTX66624 WB Image

WB analysis of various samples using GTX66624 PKC zeta antibody.
Lane1 : U-87MGHela whole cell lysate(40ug)
Lane2 : The Brain tissue lysate of Mouse(40ug)
Lane3 : The Brain tissue lysate of Rat(40ug)
Lane4 : HEK293T whole cell lysate(40ug)
Dilution : 1:500

REFERENCE

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REVIEW

There are currently no reviews for PKC zeta antibody (GTX66624). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf