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PKC zeta antibody

Anti-PKC zeta antibody used in IHC (Paraffin sections) (IHC-P). GTX66637
Anti-PKC zeta antibody used in Western Blot (WB). GTX66637
Anti-PKC zeta antibody used in Immunoprecipitation (IP). GTX66637
Anti-PKC zeta antibody used in Western Blot (WB). GTX66637

Cat No. GTX66637

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:200
IP 1:50-1:200
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids 520-570 of Human PKC ζ.

Purification

Purified by antigen-affinity chromatography
From serum

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase C zeta , PKC-ZETA , PKC2

Cellular Localization

Cytoplasm,Endosome,Cell junction,Membrane

Background

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PKC zeta antibody used in IHC (Paraffin sections) (IHC-P). GTX66637

GTX66637 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX66637 PKC zeta antibody.
Dilution : 1:50

Anti-PKC zeta antibody used in Western Blot (WB). GTX66637

GTX66637 WB Image

WB analysis of various samples using GTX66637 PKC zeta antibody.
Lane1 : U87-MG whole cell lysate(40ug)
Lane2 : 786-O whole cell lysate(40ug)
Lane3 : The Brain tissue lysate of Mouse(40ug)
Lane4 : The Brain tissue lysate of Rat(40ug)
Dilution : 1:500

Anti-PKC zeta antibody used in Immunoprecipitation (IP). GTX66637

GTX66637 IP Image

IP analysis of mouse lung tissue lysate using GTX66637 PKC zeta antibody (Lane 1 and 2) and nonspecific IgG control antibody (Lane 3 and 4) compared to the input control (Lane 5).
Upward arrow : supernatant
Downward arrow : Immunoprecipitants

Anti-PKC zeta antibody used in Western Blot (WB). GTX66637

GTX66637 WB Image

WB analysis of various samples using GTX66637 PKC zeta antibody.
Lane1 : The Testis tissue lysate of Mouse(40ug)
Lane2 : PC12 whole cell lysate(40ug)
Lane3 : A549 whole cell lysate(40ug)
Lane4 : U-87MG whole cell lysate(40ug)
Dilution : 1:2000

REFERENCE

There are currently no references for PKC zeta antibody (GTX66637). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for PKC zeta antibody (GTX66637). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf