APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:1000 |
| 1:50-1:200 |
| 1:50-1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide, corresponding to amino acids 520-570 of Human PKC ζ.
Purification
Purified by antigen-affinity chromatography
From serum
Purity
> 95% (by SDS-PAGE)
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
protein kinase C zeta , PKC-ZETA , PKC2
Cellular Localization
Cytoplasm,Endosome,Cell junction,Membrane
Background
Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX66637 WB Image
WB analysis of various samples using GTX66637 PKC zeta antibody.
Lane1 : U87-MG whole cell lysate(40ug)
Lane2 : 786-O whole cell lysate(40ug)
Lane3 : The Brain tissue lysate of Mouse(40ug)
Lane4 : The Brain tissue lysate of Rat(40ug)
Dilution : 1:500
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GTX66637 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX66637 PKC zeta antibody.
Dilution : 1:50
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GTX66637 IP Image
IP analysis of mouse lung tissue lysate using GTX66637 PKC zeta antibody (Lane 1 and 2) and nonspecific IgG control antibody (Lane 3 and 4) compared to the input control (Lane 5).
Upward arrow : supernatant
Downward arrow : Immunoprecipitants
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GTX66637 WB Image
WB analysis of various samples using GTX66637 PKC zeta antibody.
Lane1 : The Testis tissue lysate of Mouse(40ug)
Lane2 : PC12 whole cell lysate(40ug)
Lane3 : A549 whole cell lysate(40ug)
Lane4 : U-87MG whole cell lysate(40ug)
Dilution : 1:2000
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REFERENCE
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REVIEW
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