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PKC zeta (phospho Thr410) antibody

Orthogonal
Anti-PKC zeta (phospho Thr410) antibody used in IHC (Paraffin sections) (IHC-P). GTX133939
Anti-PKC zeta (phospho Thr410) antibody used in IHC (Paraffin sections) (IHC-P). GTX133939
Anti-PKC zeta (phospho Thr410) antibody used in Western Blot (WB). GTX133939
Anti-PKC zeta (phospho Thr410) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133939

Cat. No. GTX133939

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PKC zeta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Zebrafish, Rabbit, Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr410 of human PKC zeta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887142

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase C zeta , PKC-ZETA , PKC2

Cellular Localization

Cytoplasm , Endosome

Background

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PKC zeta (phospho Thr410) antibody used in IHC (Paraffin sections) (IHC-P). GTX133939

GTX133939 IHC-P Image

PKC zeta (phospho Thr410) antibody detects PKC zeta (phospho Thr410) protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
PKC zeta (phospho Thr410) stained by PKC zeta (phospho Thr410) antibody (GTX133939) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PKC zeta (phospho Thr410) antibody used in IHC (Paraffin sections) (IHC-P). GTX133939

GTX133939 IHC-P Image

PKC zeta (phospho Thr410) antibody detects PKC zeta (phospho Thr410) protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
PKC zeta (phospho Thr410) stained by PKC zeta (phospho Thr410) antibody (GTX133939) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PKC zeta (phospho Thr410) antibody used in Western Blot (WB). GTX133939

GTX133939 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC zeta (phospho Thr410) antibody (GTX133939) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PKC zeta (phospho Thr410) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133939

GTX133939 ICC/IF Image

PKC zeta (phospho Thr410) antibody detects PKC zeta (phospho Thr410) protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PKC zeta (phospho Thr410) stained by PKC zeta (phospho Thr410) antibody (GTX133939) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169