PKC zeta (phospho Thr410) antibody

Cat. No. GTX66730

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($429)

See all PKC zeta products

Datasheet
Document

View product citations for antibody GTX66730 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:1000
IHC-P	1:50-1:200

Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic phosphopeptide derived from human PKC ζ around the phosphorylation site of Threonine 410.

Purification

Purified by antigen-affinity chromatography
From serum

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase C zeta , PKC-ZETA , PKC2

Cellular Localization

Cytoplasm,Endosome,Cell junction,Membrane

Background

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Gene ID: 5590 PRKCZ
UniProt: Q05513 PRKCZ

Research Area

DATA IMAGES

GTX66730 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX66730 PKC zeta (phospho Thr410) antibody.
Dilution : 1:100

GTX66730 WB Image

WB analysis of various samples using GTX66730 PKC zeta (phospho Thr410) antibody.
Lane1 : MCF-7 whole cell lysate
Lane2 : MCF-7 treated with insulin(100nM/ml,15 minutes) whole cell lysate
Lane3 : The Kidney tissue lysate of Rat
Lane4 : The Lung tissue lysate of Mouse
Dilution : 1:500

REFERENCE

There are currently no references for PKC zeta (phospho Thr410) antibody (GTX66730). Be the first to share your publications with this product.

Submit a Reference

REVIEW

There are currently no reviews for PKC zeta (phospho Thr410) antibody (GTX66730). Be the first to share your experience with this product.

Submit a Review

SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 429

Qty