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PKD2 antibody [C1C3]

Anti-PKD2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102212
Anti-PKD2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX102212
Anti-PKD2 antibody [C1C3] used in Western Blot (WB). GTX102212
Anti-PKD2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX102212

Cat No. GTX102212

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all PKD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

Molt4

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKD2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase D2 , HSPC187 , PKD2 , nPKC-D2

Background

The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PKD2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102212

GTX102212 ICC/IF Image

PKD2 antibody [C1C3] detects PKD2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PKD2 protein stained by PKD2 antibody [C1C3] (GTX102212) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-PKD2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX102212

GTX102212 IHC-P Image

PKD2 antibody [C1C3] detects PKD2 protein at cytosol and membrane on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
PKD2 antibody [C1C3] (GTX102212) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PKD2 antibody [C1C3] used in Western Blot (WB). GTX102212

GTX102212 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PKD2 antibody [C1C3] (GTX102212) diluted at 1:500.

Anti-PKD2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX102212

GTX102212 IHC-P Image

PKD2 antibody [C1C3] detects PKD2 protein at cytosol and membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
PKD2 antibody [C1C3] (GTX102212) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Tandon M et al. PLoS One 2015; 10 (3) : e0119346 SD-208, a novel protein kinase D inhibitor, blocks prostate cancer cell proliferation and tumor growth in vivo by inducing G2/M cell cycle arrest.
Application : IP
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169