Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PKD2 antibody [C1C3] (GTX102212) diluted at 1:500.

PKD2 antibody [C1C3] detects PKD2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PKD2 protein stained by PKD2 antibody [C1C3] (GTX102212) diluted at 1:200.
Blue: Hoechst 33342 staining.

PKD2 antibody [C1C3] detects PKD2 protein at cytosol and membrane on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
PKD2 antibody [C1C3] (GTX102212) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

PKD2 antibody [C1C3] detects PKD2 protein at cytosol and membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
PKD2 antibody [C1C3] (GTX102212) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

The data was published in the journal PLoS One in 2015. PMID: 25747583