PKD2 antibody [C1C3]

Name: PKD2 antibody [C1C3]
Brand: GeneTex
SKU: GTX102212
Price: 329.00 USD
Rating: 5 (1 reviews)

Anti-PKD2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102212

Anti-PKD2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX102212

Anti-PKD2 antibody [C1C3] used in Western Blot (WB). GTX102212

Cat No. GTX102212

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse
Package	100 μl ($329), 25 μl ($169)

See all PKD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	Assay dependent

Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

Molt4

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKD2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951437

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase D2 , HSPC187 , PKD2 , nPKC-D2

Background

The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Gene ID: 25865 PRKD2
UniProt: Q9BZL6 PRKD2

Research Area

DATA IMAGES

GTX102212 ICC/IF Image

PKD2 antibody [C1C3] detects PKD2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PKD2 protein stained by PKD2 antibody [C1C3] (GTX102212) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX102212 IHC-P Image

PKD2 antibody [C1C3] detects PKD2 protein at cytosol and membrane on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
PKD2 antibody [C1C3] (GTX102212) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102212 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PKD2 antibody [C1C3] (GTX102212) diluted at 1:500.

GTX102212 IHC-P Image

PKD2 antibody [C1C3] detects PKD2 protein at cytosol and membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
PKD2 antibody [C1C3] (GTX102212) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Tandon M et al. PLoS One 2015; 10 (3) : e0119346 SD-208, a novel protein kinase D inhibitor, blocks prostate cancer cell proliferation and tumor growth in vivo by inducing G2/M cell cycle arrest.

Application : IP

Reactivity : Human

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