APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Recommended starting dilution is 1:1000. |
| Recommended starting dilution is 1:250. |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT1B10 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PKM4 protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
pyruvate kinase M1/2 , CTHBP , HELS30 , OIP3 , PK3 , PKM , PKM2 , TCB , THBP1 , pyruvate kinase, muscle
Cellular Localization
Cytoplasm,Nucleus
Background
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
Database
Research Area
DATA IMAGES
![Anti-PKM antibody [AT1B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57604](/templates/default/js/lightbox/images/loading.gif "Anti-PKM antibody [AT1B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57604")
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GTX57604 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57604 PKM antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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GTX57604 ICC/IF Image
ICC/IF analysis of A549 cells using GTX57604 PKM antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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GTX57604 WB Image
WB analysis of Recombinant Human PKM2 protein using GTX57604 PKM antibody.
Loading : 50 ng
Dilution : 1:1000
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GTX57604 WB Image
WB analysis of various samples using GTX57604 PKM antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : MCF-7 whole cell lysate
Lane 4 : A549 whole cell lysate
Lane 5 : 293T whole cell lysate
Dilution : 1:1000
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REFERENCE
There are currently no references for PKM antibody [AT1B10] (GTX57604). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PKM antibody [AT1B10] (GTX57604). Be the first to share your experience with this product.
![Anti-PKM antibody [AT1B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57604](/upload/website/prouct_img/small/GTX57604/GTX57604_20200204_IF1_w_23061123_181.webp "Anti-PKM antibody [AT1B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57604")
![Anti-PKM antibody [AT1B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57604](/upload/website/prouct_img/small/GTX57604/GTX57604_20200204_IF2_w_23061123_258.webp "Anti-PKM antibody [AT1B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57604")
![Anti-PKM antibody [AT1B10] used in Western Blot (WB). GTX57604](/upload/website/prouct_img/small/GTX57604/GTX57604_20200204_WB2_w_23061123_758.webp "Anti-PKM antibody [AT1B10] used in Western Blot (WB). GTX57604")
![Anti-PKM antibody [AT1B10] used in Western Blot (WB). GTX57604](/upload/website/prouct_img/small/GTX57604/GTX57604_20200204_WB1_w_23061123_443.webp "Anti-PKM antibody [AT1B10] used in Western Blot (WB). GTX57604")
![Anti-PKM antibody [AT1B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57604](/upload/website/prouct_img/normal/GTX57604/GTX57604_20200204_IF1_w_23061123_181.webp "Anti-PKM antibody [AT1B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57604")
![Anti-PKM antibody [AT1B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57604](/upload/website/prouct_img/normal/GTX57604/GTX57604_20200204_IF2_w_23061123_258.webp "Anti-PKM antibody [AT1B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57604")
![Anti-PKM antibody [AT1B10] used in Western Blot (WB). GTX57604](/upload/website/prouct_img/normal/GTX57604/GTX57604_20200204_WB2_w_23061123_758.webp "Anti-PKM antibody [AT1B10] used in Western Blot (WB). GTX57604")
![Anti-PKM antibody [AT1B10] used in Western Blot (WB). GTX57604](/upload/website/prouct_img/normal/GTX57604/GTX57604_20200204_WB1_w_23061123_443.webp "Anti-PKM antibody [AT1B10] used in Western Blot (WB). GTX57604")