*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
mouse brain , rat brain
Rabbit, Bovine, Chicken, Xenopus laevis(>80% identity)
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.
1.43 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human PKM. The exact sequence is proprietary.
Affinity purified by Protein G.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
pyruvate kinase M1/2,CTHBP,HEL-S-30,OIP3,PK3,PKM2,TCB,THBP1
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
GTX634796 ICC/IF Image
PKM antibody [GT1810] detects PKM protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PKM stained by PKM antibody [GT1810] (GTX634796) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 μm.
GTX634796 WB Image
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM antibody [GT1810] (GTX634796) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX634796 WB Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM antibody [GT1810] (GTX634796) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.