GeneTex
United States (US)

PKM antibody [GT1810]

GTX634796_43339_20181109_WB_M_R_19011519_738.jpg
GTX634796_43339_20181026_WB_19011519_489.jpg
GTX634796_43339_20181205_ICC_IF_19021719_835.jpg

Cat No. GTX634796

Host Mouse
Clonality Monoclonal
Clone Name GT1810
Isotype IgG1
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

mouse brain , rat brain
PROPERTIES

Form

Liquid

Buffer

in PBS only

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

1.43mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKM. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CTHBP antibody , OIP3 antibody , PK3 antibody , PKM antibody , PKM2 antibody , TCB antibody , THBP1 antibody , pyruvate kinase, muscle antibody

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq]

Research Area

DATA IMAGES
GTX634796_43339_20181109_WB_M_R_19011519_738.jpg

GTX634796 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM antibody [GT1810] (GTX634796) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX634796_43339_20181026_WB_19011519_489.jpg

GTX634796 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM antibody [GT1810] (GTX634796) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX634796_43339_20181205_ICC_IF_19021719_835.jpg

GTX634796 ICC/IF Image

PKM antibody [GT1810] detects PKM protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PKM stained by PKM antibody [GT1810] (GTX634796) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.