GeneTex
United States (US)

PKM antibody

GTX107681_40009_20160616_WB_shRNA_watermark_19011507_225.jpg
GTX107681_40009_WB_M_brain_19011506_219.jpg
GTX107681_WB_19011506_777.jpg
GTX107681_40009_WB_R_muscle_19011506_488.jpg
GTX107681_IHC_19011507_873.jpg
GTX107681_40009_20181026_WB_B_19011519_713.jpg

Cat No. GTX107681

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain , rat brain

Predict Reactivity

Rabbit, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (muscle). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pyruvate Kinase M1/2,Cthbp,Hel-S-30,Oip3,Pk3,Pkm2,Tcb,Thbp1,Pkm

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107681_40009_20160616_WB_shRNA_watermark_19011507_225.jpg

GTX107681 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107681) diluted at 1:500.

GTX107681_40009_WB_M_brain_19011506_219.jpg

GTX107681 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX107681 diluted at 1:1000

GTX107681_WB_19011506_777.jpg

GTX107681 WB Image

Sample(30μg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: Hep G2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
7.5% SDS PAGE
GTX107681 diluted at 1:1000

GTX107681_40009_WB_R_muscle_19011506_488.jpg

GTX107681 WB Image

Pyruvate Kinase (muscle) antibody detects PKM2 protein by Western blot analysis.
A. 50 µg Rat brain lysate/extract
7.5 % SDS-PAGE
Pyruvate Kinase (muscle) antibody (GTX107681) dilution: 1:1000

GTX107681_IHC_19011507_873.jpg

GTX107681 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD1 xenograft , using Pyruvate Kinase(muscle)(GTX107681) antibody at 1:100 dilution.

GTX107681_40009_20181026_WB_B_19011519_713.jpg

GTX107681 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107681) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf