APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain , rat brain
Predict Reactivity
Rabbit, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.22 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (muscle). The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Pyruvate Kinase M1/2,Cthbp,Hel-S-30,Oip3,Pk3,Pkm2,Tcb,Thbp1,Pkm
Cellular Localization
Cytoplasm , Nucleus
Background
The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX107681 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107681) diluted at 1:500.
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GTX107681 WB Image
Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX107681 diluted at 1:1000
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GTX107681 WB Image
Sample(30μg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: Hep G2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
7.5% SDS PAGE
GTX107681 diluted at 1:1000
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GTX107681 WB Image
Pyruvate Kinase (muscle) antibody detects PKM2 protein by Western blot analysis.
A. 50 µg Rat brain lysate/extract
7.5 % SDS-PAGE
Pyruvate Kinase (muscle) antibody (GTX107681) dilution: 1:1000
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GTX107681 IHC-P Image
Immunohistochemical analysis of paraffin-embedded DLD1 xenograft , using Pyruvate Kinase(muscle)(GTX107681) antibody at 1:100 dilution.
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GTX107681 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107681) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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