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PKM antibody

Anti-PKM antibody used in Western Blot (WB). GTX107977
Anti-PKM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107977
Anti-PKM antibody used in Western Blot (WB). GTX107977
Anti-PKM antibody used in Immunoprecipitation (IP). GTX107977
Anti-PKM antibody used in Western Blot (WB). GTX107977
Anti-PKM antibody used in Western Blot (WB). GTX107977
Anti-PKM antibody used in IHC (Paraffin sections) (IHC-P). GTX107977
Anti-PKM antibody used in Western Blot (WB). GTX107977

Cat No. GTX107977

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain , rat brain

Predict Reactivity

Rabbit, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (muscle). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

pyruvate kinase M1/2 , CTHBP , HEL-S-30 , OIP3 , PK3 , PKM2 , TCB , THBP1

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-PKM antibody used in Western Blot (WB). GTX107977

GTX107977 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107977) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PKM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107977

GTX107977 ICC/IF Image

Pyruvate Kinase (muscle) antibody detects Pyruvate Kinase (muscle) protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Pyruvate Kinase (muscle) protein stained by Pyruvate Kinase (muscle) antibody (GTX107977) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-PKM antibody used in Western Blot (WB). GTX107977

GTX107977 WB Image

Pyruvate Kinase (muscle) antibody detects Pyruvate Kinase (muscle) protein by Western blot analysis. Rat tissue extracts (50 μg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with Pyruvate Kinase (muscle) antibody (GTX107977) diluted by 1:2000.

Anti-PKM antibody used in Immunoprecipitation (IP). GTX107977

GTX107977 IP Image

Immunoprecipitation of PKM2 protein from 293T whole cell extracts using 5 μg of Pyruvate Kinase (muscle) antibody (GTX107977).
Western blot analysis was performed using Pyruvate Kinase (muscle) antibody (GTX107977).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-PKM antibody used in Western Blot (WB). GTX107977

GTX107977 WB Image

Pyruvate Kinase (muscle) antibody detects Pyruvate Kinase (muscle) protein by Western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with Pyruvate Kinase (muscle) antibody (GTX107977) diluted by 1:2000.

Anti-PKM antibody used in Western Blot (WB). GTX107977

GTX107977 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107977) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-PKM antibody used in IHC (Paraffin sections) (IHC-P). GTX107977

GTX107977 IHC-P Image

Pyruvate Kinase (muscle) antibody detects Pyruvate Kinase (muscle) protein at cytosol on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
Pyruvate Kinase (muscle) antibody (GTX107977) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PKM antibody used in Western Blot (WB). GTX107977

GTX107977 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107977) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

PKM antibody Cat No. GTX107977

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 08-Jan-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : PANC1 cells
Amount used : 25 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169