GeneTex
United States (US)

PKM antibody

KO/KD Overexpression
GTX107977_41759_20160616_WB_shRNA_watermark_19011507_898.jpg
GTX107977_40016_20150410_IFA_19011507_865.jpg
GTX107977_41766_WB_R_brain_19011507_996.jpg
GTX107977_41766_WB_19011507_140.jpg
GTX107977_40016_20141231_IP_19011507_367.jpg
GTX107977_41766_WB_M_brain_19011507_328.jpg
GTX107977_41766_20181026_WB_B_19011519_332.jpg
GTX107977_40016_IHC_19011507_925.jpg

Cat No. GTX107977

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain , rat brain

Predict Reactivity

Rabbit, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (muscle). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CTHBP antibody, MGC3932 antibody, OIP3 antibody, PK3 antibody, PKM antibody, TCB antibody, THBP1 antibody, PKM2 antibody, PK, muscle type antibody, tumor M2-PK antibody, OIP-3 antibody, pyruvate kinase 2/3 antibody, pyruvate kinase muscle isozyme antibody, OPA-interacting protein 3 antibody, p58 antibody, pyruvate kinase isozymes M1/M2 antibody, thyroid hormone-binding protein 1 antibody, cytosolic thyroid hormone-binding protein antibody, thyroid hormone-binding protein, cytosolic antibody, pyruvate kinase, muscle antibody

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107977_41759_20160616_WB_shRNA_watermark_19011507_898.jpg

GTX107977 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107977) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107977_40016_20150410_IFA_19011507_865.jpg

GTX107977 ICC/IF Image

Pyruvate Kinase (muscle) antibody detects Pyruvate Kinase (muscle) protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Pyruvate Kinase (muscle) protein stained by Pyruvate Kinase (muscle) antibody (GTX107977) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX107977_41766_WB_R_brain_19011507_996.jpg

GTX107977 WB Image

Pyruvate Kinase (muscle) antibody detects Pyruvate Kinase (muscle) protein by Western blot analysis. Rat tissue extracts (50 μg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with Pyruvate Kinase (muscle) antibody (GTX107977) diluted by 1:2000.

GTX107977_41766_WB_19011507_140.jpg

GTX107977 WB Image

Pyruvate Kinase (muscle) antibody detects Pyruvate Kinase (muscle) protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Pyruvate Kinase (muscle) antibody (GTX107977) diluted by 1:1000.

GTX107977_40016_20141231_IP_19011507_367.jpg

GTX107977 IP Image

Immunoprecipitation of PKM2 protein from 293T whole cell extracts using 5 μg of Pyruvate Kinase (muscle) antibody (GTX107977).
Western blot analysis was performed using Pyruvate Kinase (muscle) antibody (GTX107977).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX107977_41766_WB_M_brain_19011507_328.jpg

GTX107977 WB Image

Pyruvate Kinase (muscle) antibody detects Pyruvate Kinase (muscle) protein by Western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with Pyruvate Kinase (muscle) antibody (GTX107977) diluted by 1:2000.

GTX107977_41766_20181026_WB_B_19011519_332.jpg

GTX107977 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107977) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107977_40016_IHC_19011507_925.jpg

GTX107977 IHC-P Image

Pyruvate Kinase (muscle) antibody detects Pyruvate Kinase (muscle) protein at cytosol on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
Pyruvate Kinase (muscle) antibody (GTX107977) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

PKM antibody Cat No. GTX107977

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 08-Jan-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : PANC1 cells
Amount used : 25 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf