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PKM antibody

KO/KD Orthogonal Overexpression
Anti-PKM antibody used in Western Blot (WB). GTX107977
Anti-PKM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107977
Anti-PKM antibody used in Western Blot (WB). GTX107977
Anti-PKM antibody used in Immunoprecipitation (IP). GTX107977
Anti-PKM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107977
Anti-PKM antibody used in IHC (Paraffin sections) (IHC-P). GTX107977
Anti-PKM antibody used in Western Blot (WB). GTX107977
Anti-PKM antibody used in Western Blot (WB). GTX107977
Anti-PKM antibody used in Western Blot (WB). GTX107977

Cat. No. GTX107977

Reference 8
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PKM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

293T , PKM-transfected 293T

Predict Reactivity

Rabbit, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.13 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951264

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

pyruvate kinase M1/2 , CTHBP , HEL-S-30 , OIP3 , PK3 , PKM2 , TCB , THBP1

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PKM antibody used in Western Blot (WB). GTX107977

GTX107977 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107977) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PKM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107977

GTX107977 ICC/IF Image

Pyruvate Kinase (muscle) antibody detects Pyruvate Kinase (muscle) protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Pyruvate Kinase (muscle) protein stained by Pyruvate Kinase (muscle) antibody (GTX107977) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-PKM antibody used in Western Blot (WB). GTX107977

GTX107977 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107977) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-PKM antibody used in Immunoprecipitation (IP). GTX107977

GTX107977 IP Image

Immunoprecipitation of PKM protein from 293T whole cell extracts using 5 μg of PKM antibody (GTX107977).
Western blot analysis was performed using PKM antibody (GTX107977).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

Anti-PKM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107977

GTX107977 ICC/IF Image

PKM antibody detects PKM protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PKM stained by PKM antibody (GTX107977) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PKM antibody used in IHC (Paraffin sections) (IHC-P). GTX107977

GTX107977 IHC-P Image

Pyruvate Kinase (muscle) antibody detects Pyruvate Kinase (muscle) protein at cytosol on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
Pyruvate Kinase (muscle) antibody (GTX107977) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PKM antibody used in Western Blot (WB). GTX107977

GTX107977 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107977) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PKM antibody used in Western Blot (WB). GTX107977

GTX107977 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107977) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PKM antibody used in Western Blot (WB). GTX107977

GTX107977 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107977) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Kerrie-Ann McMahon et al. Elife 2021; 10 Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response.
Application : WB
Reactivity : Human
Liang YC et al. Am J Pathol 2021; (Epub) Centromere Protein A (CENPA) Regulates Metabolic Reprogramming in the Colon Cancer Cells by Transcriptionally Activating Karyopherin Subunit?Alpha 2 (KPNA2).
Reactivity : Human
Mattice AMS et al. PeerJ 2018; 6 : e4918 Characterization of Pyruvate Kinase From the Anoxia Tolerant Turtle, Trachemys Scripta elegans: A Potential Role for Enzyme Methylation During Metabolic Rate Depression.
Burman A et al. JCI Insight 2018; 3 (16) Localized hypoxia links ER stress to lung fibrosis through induction of C/EBP homologous protein.
Application : IHC-P
Reactivity : Human
Chang SJ et al. Arch Biochem Biophys 2018; 647 : 10-32 Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

PKM antibody Cat. No. GTX107977

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-PKM antibody used in Western Blot (WB). GTX107977
Date : Anonymous submitted on 08-Jan-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : PANC1 cells
Amount used : 25 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Submit a Review
Application Reference
Kerrie-Ann McMahon et al. Elife 2021; 10 Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response.
Application : WB
Reactivity : Human
Liang YC et al. Am J Pathol 2021; (Epub) Centromere Protein A (CENPA) Regulates Metabolic Reprogramming in the Colon Cancer Cells by Transcriptionally Activating Karyopherin Subunit?Alpha 2 (KPNA2).
Reactivity : Human
Mattice AMS et al. PeerJ 2018; 6:e4918 Characterization of Pyruvate Kinase From the Anoxia Tolerant Turtle, Trachemys Scripta elegans: A Potential Role for Enzyme Methylation During Metabolic Rate Depression.
Burman A et al. JCI Insight 2018; 3 (16) Localized hypoxia links ER stress to lung fibrosis through induction of C/EBP homologous protein.
Application : IHC-P
Reactivity : Human
Chang SJ et al. Arch Biochem Biophys 2018; 647:10-32 Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma.
Application : WB
Reactivity : Human
Jan CI et al. Int J Biol Sci? 2016; 12 (7):786-798 Fenofibrate Suppresses Oral Tumorigenesis via Reprogramming Metabolic Processes: Potential Drug Repurposing for Oral Cancer.
Application : WB
Reactivity : Human
May EW et al. Pharmacol Res 2014; (Epub) identification of up- and down-regulated proteins in *-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of *-associated resistance.
Application : WB
Reactivity : Human
Filiou M D et al. J Psychiatr Res 2014; (Epub) Behavioral extremes of trait anxiety in mice are characterized by distinct metabolic profiles
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169