GeneTex
United States (US)

PKM antibody

GTX111319_40100_20160616_WB_shRNA_watermark_19011507_355.jpg
GTX111319_40100_WB_R_muscle_19011507_965.jpg
GTX111319_WB_19011507_116.jpg
GTX111319_40100_WB_M_brain_19011507_504.jpg
GTX111319_IHC_19011507_197.jpg

Cat No. GTX111319

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

A431 , H1299 , mouse brain , rat muscle , *HeLa S3 , 293T

Predict Reactivity

Rabbit, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (muscle). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pyruvate Kinase M1/2,Cthbp,Hel-S-30,Oip3,Pk3,Pkm2,Tcb,Thbp1,Pkm

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX111319_40100_20160616_WB_shRNA_watermark_19011507_355.jpg

GTX111319 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX111319) diluted at 1:500.

GTX111319_40100_WB_R_muscle_19011507_965.jpg

GTX111319 WB Image

Pyruvate Kinase (muscle) antibody detects PKM2 protein by Western blot analysis.
A. 50 µg Rat muscle lysate/extract
7.5 % SDS-PAGE
Pyruvate Kinase (muscle) antibody (GTX111319) dilution: 1:1000

GTX111319_WB_19011507_116.jpg

GTX111319 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
7.5% SDS PAGE
GTX111319 diluted at 1:1000

GTX111319_40100_WB_M_brain_19011507_504.jpg

GTX111319 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111319 diluted at 1:1000

GTX111319_IHC_19011507_197.jpg

GTX111319 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Pyruvate Kinase(muscle)(GTX111319) antibody at 1:100 dilution.

REVIEW

PKM antibody Cat No. GTX111319

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 05-Nov-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa S3 cells
Amount used : 10μg
Blocking : TBST with 1% BSA, 25°C, 12Hr
Primary Antibody : 1:1000, 25°C, 3Hr
SDS
Glycerol.pdf
Thimerosal.pdf