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PKM antibody

KO/KD
Anti-PKM antibody used in Western Blot (WB). GTX111319
Anti-PKM antibody used in IHC (Paraffin sections) (IHC-P). GTX111319
Anti-PKM antibody used in Western Blot (WB). GTX111319
Anti-PKM antibody used in Western Blot (WB). GTX111319
Anti-PKM antibody used in Western Blot (WB). GTX111319

Cat. No. GTX111319

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PKM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

Mouse brain , Rat muscle , A431 , H1299 , 293T

Predict Reactivity

Rabbit, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951265

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pyruvate kinase M1/2 , CTHBP , HEL-S-30 , OIP3 , PK3 , PKM2 , TCB , THBP1

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PKM antibody used in Western Blot (WB). GTX111319

GTX111319 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX111319) diluted at 1:500.

Anti-PKM antibody used in IHC (Paraffin sections) (IHC-P). GTX111319

GTX111319 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Pyruvate Kinase(muscle)(GTX111319) antibody at 1:100 dilution.

Anti-PKM antibody used in Western Blot (WB). GTX111319

GTX111319 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111319 diluted at 1:1000

Anti-PKM antibody used in Western Blot (WB). GTX111319

GTX111319 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
7.5% SDS PAGE
GTX111319 diluted at 1:1000

Anti-PKM antibody used in Western Blot (WB). GTX111319

GTX111319 WB Image

Pyruvate Kinase (muscle) antibody detects PKM2 protein by Western blot analysis.
A. 50 μg Rat muscle lysate/extract
7.5 % SDS-PAGE
Pyruvate Kinase (muscle) antibody (GTX111319) dilution: 1:1000

REFERENCE

There are currently no references for PKM antibody (GTX111319). Be the first to share your publications with this product.
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REVIEW

PKM antibody Cat. No. GTX111319

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-PKM antibody used in Western Blot (WB). GTX111319
Date : Anonymous submitted on 05-Nov-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa S3 cells
Amount used : 10μg
Blocking : TBST with 1% BSA, 25°C, 12Hr
Primary Antibody : 1:1000, 25°C, 3Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169